DR7 DataRelease
Release Date: 01/01/2014
| SDY21: Gene Expression Study of TLR Responses on Dendritic Cells | |||||||
| Status: | New | ||||||
| Description: | Cytokine and gene expression profile after TLR activation of dendritic cells. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3YUM4W1N9 | ||||||
| Subjects: | 4 | ||||||
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| Publications: | None | ||||||
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| Clinical Assessments: | None | ||||||
| SDY29: Determine of anti-PA titer kinetics | |||||||
| Status: | New | ||||||
| Description: | Four groups of 12 mice will each be immunized with rPA ± Alum (alhydrogel). Two groups will then be boosted on Day 227 with rPA alone. Mice will be bled every 2-3 days over the course of 283 days. Serum samples will be prepared and assayed for anti-PA titers using ELISA and/or anthrax toxin neutralization assays. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3VDXBDPRQ | ||||||
| Subjects: | 48 | ||||||
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| Publications: | None | ||||||
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| Clinical Assessments: | None | ||||||
| SDY30: Comprehensive study of germinal center development and antibody response | |||||||
| Status: | New | ||||||
| Description: | To comprehensively study the humoral response to immunization with rPA with or without Alum using in vitro antibody analyses and tissue staining. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3THUFGISM | ||||||
| Subjects: | 54 | ||||||
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| Publications: | None | ||||||
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| Clinical Assessments: | None | ||||||
| SDY31: Determining the effect of different adjuvants and doses on the immune response | |||||||
| Status: | New | ||||||
| Description: | Inbred C57BL/6 female mice (8-10 weeks old) were immunized subcutaneously according to the 8 immunization groups. Serum samples were collected weekly from 2 staggered groups and analyzed for PA-specific (Total Ig, IgG1, IgG2a, and IgG3) and neutralizing antibody titers. Also, spleen, thymus, and brachial and inguinal lymph nodes will be harvested from 1 of each group at 4 time points for histology. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M37IXT07LU | ||||||
| Subjects: | 96 | ||||||
| Study PI, contact: |
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| Publications: | None | ||||||
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| Clinical Assessments: | None | ||||||
| SDY32: Determining the effect of different adjuvants and doses on the immune response to rPA | |||||||
| Status: | New | ||||||
| Description: | Inbred C57BL/6 female mice (12-15 weeks old) were immunized subcutaneously according to the 7 immunization groups. Serum samples were collected weekly from subgroup A and analyzed serum antibody response using ELISA, Toxin Neutralization Assays, and Surface Plasmon Resonance. Also, brachial and inguinal lymph nodes were harvested from 3 of each group at 10 time points. Lymph nodes from one side of each mouse were used for histology and nodes from the other side were used for phenotyping by flow cytometry. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3OL4ZG9JN | ||||||
| Subjects: | 210 | ||||||
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| Publications: | None | ||||||
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| Clinical Assessments: | None | ||||||
| SDY224: Immune Responses to Seasonal TIV 2010-2011 Influenza Vaccination in Humans (see companion study SDY396,SDY564) | |||||||||||||||
| Status: | New | ||||||||||||||
| Description: | High-frequency sampling combined with systems biology analysis of human peripheral blood cells following influenza vaccination was used to investigate T cell and B cell responses. Functional principal component analysis was used to examine time varying B cell vaccine response highlighting a single subject-specific mathematical pattern explaining ninety percent of the transcriptome variation. In addtition, daily sampling and monitoring of the proliferation marker Ki-67, revealed influenza-specific CD4 T cells do respond to vaccination. | ||||||||||||||
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| DOI: | 10.21430/M37KMO7JLW | ||||||||||||||
| Subjects: | 14 | ||||||||||||||
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| Clinical Assessments: | None | ||||||||||||||
| SDY256: Cooperation between DCs and basophils in TH2 response to papain | |||||||
| Status: | New | ||||||
| Description: | To investigate the contribution of dendritic cells and basophils to TH2 differentiation, induction with OVA and papain was used to investigate signalling in both in vivo and in vitro systems. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3GCSV0KAE | ||||||
| Subjects: | 211 | ||||||
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| Clinical Assessments: | None | ||||||
| SDY258: Dendritic cells and TH2 differentiation in vivo | |||||||
| Status: | New | ||||||
| Description: | TH2 cell differentiation was investigated by measuring IL-4 output after OVA plus papain challenge in normal and dendritic-cell depleted mice. The role of migrating dermal dendritic cells in OVA plus papain challenge by blocking migration and depleting dendritic cell subtypes. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3F0JEOKSX | ||||||
| Subjects: | 281 | ||||||
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| Clinical Assessments: | None | ||||||
| SDY259: Reactive oxygen species and TH2 responses to papain | |||||||
| Status: | New | ||||||
| Description: | The role of reactive oxygen species in the TH2 response to papain was investigated. The production of ROS by dendritic cells both in vivo and in vitro was examined as an inducer of TH2 differentiation and suppressor of TH1 differentiation. CD70 and IL-12 as effectors of ROS-mediated suppression of TH1 differentiation were investigated as well as the effect of ROS on IL-4-mediated TH2 response. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3DWBGXETN | ||||||
| Subjects: | 212 | ||||||
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| Clinical Assessments: | None | ||||||
| SDY260: The role of TLR4 and TRIF signaling in TH2 response to papain | |||||||
| Status: | New | ||||||
| Description: | The role of TLR4 signalling in TH2 response to papain was measured by IL-4 and antibody production. The involvement of MyD88, TRIF, and oxidized phospholipids in the TLR4 pathway was examined. Basophil recruitment to the draining lymph node through dendritic-cell-secreted CCL7 was investigated as a component of the TLR4/TRIF pathway. | ||||||
| Program/Contract: |
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| DOI: | 10.21430/M3KIQA4LOV | ||||||
| Subjects: | 110 | ||||||
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| Clinical Assessments: | None | ||||||
| SDY180: Systems scale interactive exploration reveals quantitative and qualitative differences in response to 2009-2010 Fluzone influenza vaccine and pneumococcal vaccine | |||||||||||||||||
| Status: | Updated | ||||||||||||||||
| Description: | Systems immunology approaches were employed to investigate innate and adaptive immune responses to influenza and pneumococcal vaccines. These two non-live vaccines show different magnitudes of transcriptional responses at different time points af- ter vaccination. Software solutions were developed to explore correlates of vaccine efficacy measured as antibody titers at day 28. These enabled a further dissection of transcriptional responses. Thus, the innate response, measured within hours in the peripheral blood, was dominated by an interferon transcriptional signature after influenza vaccination and by an inflammation signature after pneumo- coccal vaccination. Day 7 plasmablast responses induced by both vaccines was more pronounced after pneumococcal vaccination. Together, these results suggest that comparing global immune responses elicited by different vaccines will be critical to our understanding of the immune mechanisms underpinning successful vaccination. | ||||||||||||||||
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| DOI: | 10.21430/M3I44H8R17 | ||||||||||||||||
| Subjects: | 46 | ||||||||||||||||
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| Clinical Assessments: | None | ||||||||||||||||
| SDY241: Simulation and Prediction of the Adaptive Immune Response and Quantification of Early and Adaptive Immune Response Kinetics to Influenza A Virus Infection | ||||||||||
| Status: | Updated | |||||||||
| Description: | Seasonal and pandemic influenza A virus (IAV) continues to be a public health threat. Modeling approaches were used combined with experimental data to investigate innate and adaptive immune responses to IAV infection. Mathematical models developed describe the dynamic interactions between influenza virus, target cells, cytotoxic lymphocytes, and virus-specific IgG and IgM. A two-compartment model developed quantifies the effects of viral replication and adaptive immunity. | |||||||||
| Program/Contract: |
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| DOI: | 10.21430/M3ERWHDJEO | |||||||||
| Subjects: | 494 | |||||||||
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| Clinical Assessments: | None | |||||||||