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Introduction

ImmPort Data Upload Templates Use

This guide describes the structure and use of the ImmPort data upload templates. For each template, the purpose, structure (e.g. column headers), and data entry rules are described.

For additional information about the ImmPort Data Upload workflow, please see the ImmPort data upload user's guide and tutorials (http://immport.niaid.nih.gov/immportWeb/experimental/displayDataSubmitHome.do).

It is recommended that you open the Adobe Reader bookmarks option to see a list of the templates.

Each template includes a Schema Version tag. It is used by the ImmPort Data Package Validator and Upload Processing software to ensure the current version of the template is being used. The Schema version refers to the database schema structure and content (in particular the preferred terms and reference data). These version tags should not be modified by the user.

As a reminder, the spreadsheet versions of the templates are provided for benefit of the data providers to enable display of comments and lists in the spreadsheet. The templates uploaded to ImmPort should be saved as tab-delimited text files. Template names should match the names as they appear on the ImmPort web pages. File names are not case sensitive. However, downloading templates from a web browser may cause your computer to append a suffix to the file name and this will prevent ImmPort from recognizing a template name.

ImmPort Data Upload Packages

The ImmPort data upload workflow is intended to be incremental or modular. You can send one or a few ImmPort upload templates and associated files (e.g. protocols or results) in a data upload package ZIP archive. Or, you can send in many templates and associated files. The order of uploading templates (study before subject or subject before study?) is determined by what descriptive data is referenced within a template and therefore what dependencies exist for an uploaded template to be successfully processed. For example, successfully uploading a subject template requires that a protocol and study arm be referenced, so both the protocol and study design template need to be in the same ZIP archive as the subject template or already uploaded and processed in ImmPort. Individual files contained in the package cannot exceed 2 GBytes (2^31-1 = 2,147,483,647 bytes) in size.

ImmPort Data Model Overview

ImmPort's model for handling research data is to organize information into metadata or descriptive categories. Each category has its own template (or suite of templates). The metadata categories and their functions are as follows:

Study: Studies provide the context and organization of a research effort. Studies organize subjects into groups (e.g. arms or cohorts) based on phenotype and/or treatment. The planned visit schedule provides a guide as to the temporal relationship between samples and encounters (i.e is the sample taken before or after a treatment). If the research includes seasonally variable treatments (e.g seasonal influenza vaccine), a separate study should be created for each season.

Subjects: Subjects may be patients or animals from which samples are taken for analysis. Two .xls templates (one for human and one for animal subjects) are available for recording subject information. In these files, treatment protocols used on the subjects can also be listed as well as many other details. Subjects are assigned to a single group (arm or cohort) within a study and maybe linked to multiple studies.

Biological Samples: Describe the types of samples taken from subjects or cell culture and processed for the experiment (i.e. organs, tissue, blood, plasma, cell culture name, etc.), when the samples were taken in the course of a study and protocols used in the sample collection, processing, and/or treatment. Samples are linked to a single study.

Experiments: Describe the measurement technique of the experiment and the links to protocols used in the experiment.

Experiment Samples: The biological samples analyzed in an experiment are linked to the assay reagent, protocol and results via the experiment sample record. Several different template files are available for listing sample details for different assay approaches. An experiment sample should be linked to a single biological sample.

Treatments: Describe the experimental conditions for specific biosamples or experiment samples. Treatments link to experiment samples as well as biosamples if needed.

Protocols: Describe the methods and procedures in studies, subject recruitment/treatment, sample collection/preparation/treatment and experiments. Protocols may be PDF files, Word documents, Excel or other file types.

Reagents: Provide detailed information about the reagents used in an experiment. Since different analysis platforms employ very different reagents, several different template files are available for listing reagent details for different assay approaches.

Field Name Description
Description: The Description field provides a detailed description of the column of the Template
Required: The Required Field can either have the values Yes or No. Yes means the user must enter data for this column. No means it is optional for the user to enter data for this column.
Lookup: The Lookup Field can either have the values "None" or "Please refer to Appendix A - {Name of the Lookup table}". "None" means this column does not have a pre-defined set of values and no dropdown will be available in the template. "Please refer to Appendix A - {Name of the Lookup table}" means that this column has a pre-defined set of values and a drop-down will be available for the user to select from in the template. The user can click on the "Please refer to Appendix A-{Name of the Lookup}" link in the LookupField and it will take you to the section in Appendix A where the values for the corresponding lookup table are listed.
Comment: The Comment Field text will be displayed in the template as a comment for the corresponding column. This field provides more information on what kind of data needs to be set for the column
Database Table: The Database Table Field is the name of the database table which will store the data entered for this column by the user in the template.
Database Column: The Database Column Field is the name of the column in the ImmPort database which will store the data entered for this column by the user in the template.
Database Type: The Database Type Field is the data type of the column in the ImmPort database which will store the data entered for this column by the user in the template.

Template File Loading Order

ImmPort loads the template files in a specified loading order that is specified below in the following table.

Template File Loading Order
protocols.txt
reagents.array.txt
reagents.elisa.txt
reagents.elispot.txt
reagents.mbaa.txt
reagents.flow_cytometry.txt
reagents.hai.txt
reagents.cytof.txt
reagents.neutralizing_antibody_titer.txt
reagents.pcr.txt
reagents.sequencing.txt
reagents.virus_neutralization.txt
reagents.hla_typing.txt
reagents.kir_typing.txt
reagents.other.txt
reagent_sets.txt
treatments.txt
basic_study_design.txt
subjectanimals.txt
subjecthumans.txt
study_design_edit.txt
adverseevents.txt
interventions.txt
assessments.txt
biosamples.txt
labtestpanels.txt
labtests.txt
labtest_results.txt
experiments.txt
controlsamples.txt
standardcurves.txt
experimentsamples.flow_cytometry.txt
experimentsamples.cytof.txt
experimentsamples.gene_expression_array.txt
experimentsamples.genotyping_array.txt
experimentsamples.hla.txt
experimentsamples.image_histology.txt
experimentsamples.kir.txt
experimentsamples.mbaa.txt
experimentsamples.rna_sequencing.txt
experimentsamples.other.txt
experimentsamples.mass_spectrometry_metabolomics.txt
experimentsamples.mass_spectrometry_proteomics.txt
experimentsamples.elisa.txt
experimentsamples.elispot.txt
experimentsamples.hai.txt
experimentsamples.virus_neutralization.txt
experimentsamples.neutralizing_antibody_titer.txt
experimentsamples.qrt-pcr.txt
immuneexposure.txt
publicrepositories.txt
elisa_results.txt
elispot_results.txt
hai_results.txt
pcr_results.txt
virus_neutralization_results.txt
hla_typing.txt
kir_typing.txt
rna_seq_results.txt
mass_spectrometry_metabolomic_results.txt
mass_spectrometry_proteomic_results.txt
mbaa_results.txt
fcm_derived_data.txt
cytof_derived_data.txt

The next section describes each data upload template.

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